Loss-of-function screening by randomized intracellular antibodies: identification of hnRNP-K as a potential target for metastasis.

Abstract excerpt

We have developed a loss-of-function screening system on the basis of intracellular expression of single domain antibodies. We demonstrate its use in identification of potential targets of metastasis of human cancerous cells. Randomized intracellular antibodies were expressed in highly metastatic cells, and a derivative pool of cells with loss of migration phenotype in chemotaxis assay was isolated. Isolation of...

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