Article
Enhancement of human osteoblast proliferation and phenotypic expression when cultured in human serum.
Acta orthopaedica Scandinavica - 1 Aug 2001
Hankey D P, McCabe R E, Doherty M J, Nolan P C, McAlinden M G, Nelson J, Wilson D J
Abstract excerpt
Traditionally, culture medium is supplemented with foetal bovine serum (FBS). However, in cultures of osteoblasts intended for human re-implantation, such serum presents potential risks of foreign protein contamination and transmission of viral or prion-related material, if used. We cultured human osteoblasts from 16 patients in 10% autologous human serum, 10% pooled human serum, 10% FBS or 2% Ultroser G....
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