Article
Morphologic characterization of osteoblast-like cell cultures isolated from newborn rat calvaria.
Calcified tissue international - 1 Aug 1990
Masquelier D, Herbert B, Hauser N, Mermillod P, Schonne E, Remacle C
Abstract excerpt
Two methods for harvesting osteoblast-like cell populations from newborn (10 days) rat calvaria were compared. The first one consisted in culturing the periosteum-free bones and then trypsinizing the cells on the bone surface. The second one involved the migration of the osteoblasts on glass frag...
Topics
- Alkaline Phosphatase
- Animals
- Animals, Newborn
- Cell Separation
- Cells, Cultured
- Histocytochemistry
- Microscopy, Electron
- Microscopy, Electron, Scanning
- Osteoblasts
- Phenotype
- Rats
- Rats, Inbred Strains
- Skull
- Trypsin