Article
Morphologic characterization of osteoblast-like cell cultures isolated from newborn rat calvaria.
Calcified tissue international - 1 Aug 1990
Masquelier D, Herbert B, Hauser N, Mermillod P, Schonne E, Remacle C
Abstract excerpt
Two methods for harvesting osteoblast-like cell populations from newborn (10 days) rat calvaria were compared. The first one consisted in culturing the periosteum-free bones and then trypsinizing the cells on the bone surface. The second one involved the migration of the osteoblasts on glass fragments before trypsinization. Since the plating efficiency, the proportion of alkaline phosphatase-positive cells, the...
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