Screen for dominant behavioral mutations caused by genomic insertion of P-element transposons in Drosophila: an examination of the integration of viral vector sequences.

Abstract excerpt

Here we report the development of a high-throughput screen to assess dominant mutation rates caused by P-element transposition within the Drosophila genome that is suitable for assessing the undesirable effects of integrating foreign regulatory sequences (viral cargo) into a host genome. Three different behavioral paradigms were used: sensitivity to mechanical stress, response to heat stress, and ability to fly....

Topics

• Animals
• Animals, Genetically Modified
• Behavior, Animal
• Breeding
• Cytomegalovirus
• DNA Transposable Elements
• Dependovirus
• Drosophila
• Genes, Dominant
• Genes, Viral
• Genetic Vectors
• Mutagenesis, Insertional
• Mutation