Article
A method to invert DNA segments of the Bacillus subtilis 168 genome by recombination between two homologous sequences.
Bioscience, biotechnology, and biochemistry - 1 May 1996
Toda T, Tanaka T, Itaya M
Abstract excerpt
We developed a method that allows rapid isolation of mutant Bacillus subtilis 168 carrying an inversion of a specific DNA segment of the genome. Two incomplete neomycin resistance gene cassettes were integrated at both ends of the 1652-kb segment to be inverted. Reciprocal recombination within th...
Topics
- Anti-Bacterial Agents
- Bacillus subtilis
- Blotting, Southern
- Chromosome Inversion
- Chromosomes, Bacterial
- DNA Restriction Enzymes
- DNA, Bacterial
- Drug Resistance, Microbial
- Genome, Bacterial
- Mutagenesis, Insertional
- Mutation
- Neomycin
- Recombination, Genetic
- Sequence Homology, Nucleic Acid