Article
A method to invert DNA segments of the Bacillus subtilis 168 genome by recombination between two homologous sequences.
Bioscience, biotechnology, and biochemistry - 1 May 1996
Toda T, Tanaka T, Itaya M
Abstract excerpt
We developed a method that allows rapid isolation of mutant Bacillus subtilis 168 carrying an inversion of a specific DNA segment of the genome. Two incomplete neomycin resistance gene cassettes were integrated at both ends of the 1652-kb segment to be inverted. Reciprocal recombination within the 590-bp homologous region of these two cassettes created an intact neomycin resistance gene with concomitant inversion...
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