Article
First evidence for homologous recombination-mediated large DNA inversion on the Bacillus subtilis 168 chromosome.
Bioscience, biotechnology, and biochemistry - 1 Oct 1994
Itaya M
Abstract excerpt
A Bacillus subtilis 168 strain carrying an inversion of about 1600 kb-long chromosomal DNA was isolated. Physical and genetic analyses demonstrated that the inversion was generated as a result of homologous recombination between two homologous sequences integrated at the met and leuB loci. This is the first clear evidence of a large stable chromosomal inversion induced by homologous recombination in B. subtilis.
Topics
- Bacillus subtilis
- Blotting, Southern
- Chromosome Inversion
- Chromosomes, Bacterial
- DNA, Bacterial
- Drug Resistance, Microbial
- Genes, Bacterial
- In Situ Hybridization
- Introns
- Mutation
- Plasmids