Article
Effect of surface histidine mutations and their number on the partitioning and refolding of recombinant human granulocyte-colony stimulating factor (Cys17Ser) in aqueous two-phase systems containing chelated metal ions.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 1 Jun 2007
Zaveckas Mindaugas, Zvirbliene Aurelija, Zvirblis Gintautas, Chmieliauskaite Valerija, Bumelis Vladas, Pesliakas Henrikas
Abstract excerpt
High-level expression of recombinant proteins in Escherichia coli frequently leads to the formation of insoluble protein aggregates, termed inclusion bodies. In order to recover a native protein from inclusion bodies, various protein refolding techniques have been developed. Column-based refoldin...
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