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Article

Phosphomimetic mutation of Ser-187 of SNAP-25 increases both syntaxin binding and highly Ca2+-sensitive exocytosis.

The Journal of general physiology - 1 Mar 2007

Yang Yan, Craig Tim J, Chen Xiaohui, Ciufo Leonora F, Takahashi Masami, Morgan Alan, Gillis Kevin D

Abstract excerpt

The phosphorylation targets that mediate the enhancement of exocytosis by PKC are unknown. PKC phosporylates the SNARE protein SNAP-25 at Ser-187. We expressed mutants of SNAP-25 using the Semliki Forest Virus system in bovine adrenal chromaffin cells and then directly measured the Ca2+ dependence of exocytosis using photorelease of caged Ca2+ together with patch-clamp capacitance measurements. A flash of UV...

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Source

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Identifiers

PMID: 17325194
DOI: 10.1085/jgp.200609685
PMCID: PMC2151612

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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