Identification of two new genetically active regions associated with the osmZ locus of Escherichia coli: role in regulation of proU expression and mutagenic effect of cya, the structural gene for adenylate cyclase.

Abstract excerpt

The Escherichia coli K-12 gene coding for the nucleoid-associated protein HNS was cloned together with 5.6 kb of downstream DNA in the vector pACYC184. The cloned DNA complemented a mutation in the osmZ locus of E. coli, which codes for HNS. However, the multicopy plasmid harboring the cloned sequence was found to be mutagenic and to produce at high frequency mutations that mapped to the E. coli cya gene, which...

Topics

• Adenylyl Cyclases
• Arabinose
• Bacterial Outer Membrane Proteins
• Bacterial Proteins
• Bacteriophages
• Chromosome Mapping
• Cloning, Molecular
• DNA-Binding Proteins
• Escherichia coli
• Gene Expression Regulation, Bacterial