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Article

Isolation and characterization of conditional alleles of the yeast SIR2 gene.

Journal of molecular biology - 13 Apr 2007

Hickman Merrit, McCullough Kalyani, Woike Adrienne, Raducha-Grace Laura, Rozario Tania, Dula Mary Lou, Anderson Erica, Margalit Danielle, Holmes Scott G

Abstract excerpt

Sir2 is a protein deacetylase that mediates transcriptional silencing at the HM loci, telomeres, and rDNA repeats in yeast. To identify functionally significant regions of the Sir2 protein, we have characterized two types of mutations. First, we used random mutagenesis to create temperature-sensitive alleles of the SIR2 gene. Mutations conferring conditional silencing can be isolated throughout the SIR2 gene,...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 17316680
DOI: 10.1016/j.jmb.2007.01.044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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