Article
Evidence that two naturally occurring human insulin receptor alpha-subunit variants are immunologically distinct.
Diabetes - 1 Jan 1992
Sesti G, Marini M A, Montemurro A, Condorelli L, Borboni P, Haring H U, Ullrich A, Goldfine I D, De Pirro R, Lauro R
Abstract excerpt
The IgG from a patient (Italy 2 [I2]) with hypoglycemia, due to autoantibodies to the insulin receptor, was purified on protein A Sepharose into two fractions that were tested in various human tissues and cells. The IgG fraction that bound protein A (absorbed IgG [IgGa]) nearly completely inhibited the binding of 125I-labeled insulin to various cells or tissues (placenta, IM-9, adipocytes, HEp-2-larynx cells,...
Topics
- Adipose Tissue
- Antibodies, Monoclonal
- Cell Line
- Cell Membrane
- Female
- Genetic Variation
- Humans
- Hypoglycemia
- Immunoglobulin G
- Insulin
- Insulin-Like Growth Factor I