Article
Preference for IgG mAb binding insulin in solution or on surfaces is related to immunoglobulin variable region structures.
Journal of autoimmunity - 1 Dec 1997
Tikhomirov O Y, Thomas J W
Abstract excerpt
Studies on insulin autoantibodies often show a lack of correlation between enzyme-linked immunosorbent assays (ELISAs) and fluid phase radioimmunoassays (RIAs). Similarly, a set of IgG anti-insulin monoclonal antibodies (mAb) from BALB/c mice are found to differ in their binding in ELISAs and RIA...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Biotin
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunoglobulin Fragments
- Immunoglobulin G
- Immunoglobulin Variable Region
- Insulin
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Phenotype
- Radioimmunoassay
- Sequence Homology, Amino Acid
- Solutions