Article
Evidence that late-endosomal SNARE multimerization complex is promoted by transmembrane segments.
Biochimica et biophysica acta - 1 Mar 2007
Mascia Laura, Langosch Dieter
Abstract excerpt
Assembly of SNARE proteins into quaternary complexes is a critical step in membrane docking and fusion. Here, we have studied the influence of the transmembrane segments on formation of the late endosomal SNARE complex. The complex was assembled in vitro from full-length recombinant SNAREs and from mutants, where the transmembrane segments were either deleted or replaced by oligo-alanine sequences. We show that...
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