Article
The structural and functional implications of linked SNARE motifs in SNAP25.
Molecular biology of the cell - 1 Sept 2008
Wang Li, Bittner Mary A, Axelrod Daniel, Holz Ronald W
Abstract excerpt
We investigated the functional and structural implications of SNAP25 having two SNARE motifs (SN1 and SN2). A membrane-bound, intramolecular FRET probe was constructed to report on the folding of N-terminal SN1 and C-terminal SN2 in living cells. Membrane-bound constructs containing either or both SNARE motifs were also singly labeled with donor or acceptor fluorophores. Interaction of probes with other SNAREs...
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