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Damage to Chromosomes and DNA of Rhesus Monkey Sperm Following Cryopreservation

22 Jun 2007

Abstract excerpt

Fresh and frozen-thawed rhesus monkey sperm were analyzed for DNA damage using the comet assay and for chromosome damage by cytogenetic analysis after intracytoplasmic sperm injection (ICSI) into mouse oocytes. The percentage of fresh sperm with damaged DNA in ejaculated semen was 0 to 2.7% (n = 5). Conventional cryopreservation and storage in liquid nitrogen caused DNA damage in 25.3% to 43.7% of sperm; when...

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PMID: 17251598
DOI: 10.2164/jandrol.106.000869

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