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Article

Sonication Per Se Is Not as Deleterious to Sperm Chromosomes as Previously Inferred1

1 Jul 2000

Abstract excerpt

Although sonication is a simple way to immobilize ("kill") spermatozoa prior to injection into oocytes, this has been thought to be destructive to sperm chromosomes. Mouse and human spermatozoa were immobilized by sonication and kept in various media for up to 2 h, then their nuclei were individually injected into mouse oocytes for the analysis of chromosomes at the first cleavage metaphase. In both the mouse and...

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PMID: 10859277
DOI: 10.1095/biolreprod63.1.341

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