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Effect of sperm cryopreservation on sperm DNA stability and progeny development in rainbow trout

28 Sept 2001

Abstract excerpt

This study was carried out to test how sperm cryopreservation affected nuclear DNA stability and whether progeny development was modified when eggs were fertilized with cryopreserved spermatozoa. The "comet assay" (alkaline single-cell gel electrophoresis assay) was adapted to trout spermatozoa to estimate DNA stability as measured by alkali-induced DNA strand break formation. Because trout eggs develop in water...

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PMID: 11599051
DOI: 10.1002/mrd.1102

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