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Maintenance of genetic integrity in frozen and freeze-dried mouse spermatozoa

13 Nov 2001

Abstract excerpt

Chromosome stability was maintained in mouse spermatozoa after freeze-drying or freezing without cryoprotection in a simple Tris.HCl buffer containing EGTA (50 mM) and NaCl (50 mM). The ability of spermatozoa to activate oocytes spontaneously was not destroyed by freeze-drying or freezing without cryoprotection in this solution. Embryos derived after injecting oocytes with sperm heads from rehydrated freeze-dried...

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PMID: 11707598
DOI: 10.1073/pnas.241517598

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