Article
Detection and typing of minimal human papillomavirus DNA in plasma.
International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics - 1 Feb 2007
Wei Yu-Chi, Chou Yu-Shen, Chu Tang-Yuan
Abstract excerpt
OBJECTIVE: For clinical study of minute human papillomavirus (HPV) DNA such as those in plasma, a sensitive method that can detect a broad spectrum of HPV type is needed. METHOD: A nested polymerase chain reaction (PCR) method which utilized a consensus primer set nested to the widely utilized MY09/MY11 primer set was developed. The HPV genotype was determined by restriction digestion of the PCR product followed...
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