Detection of major HPVs by a new multiplex real-time PCR assay using type-specific primers.

doi:10.1016/j.mimet.2013.12.012

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Article

Detection of major HPVs by a new multiplex real-time PCR assay using type-specific primers.

Journal of microbiological methods - 1 Feb 2014

Şahiner Fatih, Kubar Ayhan, Yapar Mehmet, Şener Kenan, Dede Murat, Gümral Ramazan

Abstract excerpt

In this study, we aimed to develop a cost-effective, practical, and sensitive method to be used for the diagnosis of HPV infections. The presence of HPV-DNA was investigated in cervical smear samples using three different methods: MY09/11 consensus PCR, TaqMan-based type-specific real-time PCR, and SYBR Green-based multiplex PCR. Of the 315 samples, 21.6% (68/315) were HPV-DNA positive by using at least one of...

Topics

Adolescent
Adult
Aged
DNA Primers
DNA, Viral
Female
Genotype
Human Papillomavirus DNA Tests
Humans
Middle Aged
Multiplex Polymerase Chain Reaction
Papillomaviridae
Papillomavirus Infections
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity
Young Adult

Source

Open in PubMed

Identifiers

PMID: 24365111
DOI: 10.1016/j.mimet.2013.12.012

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