Article
Detection of major HPVs by a new multiplex real-time PCR assay using type-specific primers.
Journal of microbiological methods - 1 Feb 2014
Şahiner Fatih, Kubar Ayhan, Yapar Mehmet, Şener Kenan, Dede Murat, Gümral Ramazan
Abstract excerpt
In this study, we aimed to develop a cost-effective, practical, and sensitive method to be used for the diagnosis of HPV infections. The presence of HPV-DNA was investigated in cervical smear samples using three different methods: MY09/11 consensus PCR, TaqMan-based type-specific real-time PCR, and SYBR Green-based multiplex PCR. Of the 315 samples, 21.6% (68/315) were HPV-DNA positive by using at least one of...
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