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Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories

5 Jun 2007

Abstract excerpt

BACKGROUND: Human papillomavirus (HPV) genotyping is important for following up patients with persistent HPV infection and for evaluation of prevention strategy for the individual patients to be immunized with type-specific HPV vaccines. The aim of this study was to optimize a robust "low-temperature" (LoTemp) PCR system to streamline the research protocols for HPV DNA nested PCR-amplification followed by...

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PMID: 17550589
DOI: 10.1186/1750-9378-2-11

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