Article
Modifications of the fibroblast growth factor-2 gene led to a marked enhancement in secretion and stability of the recombinant fibroblast growth factor-2 protein.
Journal of cellular biochemistry - 15 Apr 2007
Chen Shin-Tai, Gysin Reinhard, Kapur Sonia, Baylink David J, Lau K-H William
Abstract excerpt
Progress in FGF-2 gene therapy has been hampered by the difficulty in achieving therapeutic levels of FGF-2 secretion. This study tested whether the addition of BMP2/4 hybrid secretion signal to the FGF-2 gene and mutation of cys-70 and cys-88 to serine and asparagine, respectively, would increase the stability and secretion of active FGF-2 protein in mammalian cells using MLV-based vectors. Single or double...
Topics
- Animals
- Blotting, Western
- Bone Morphogenetic Proteins
- Cell Line, Tumor
- Cell Proliferation
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Fibroblast Growth Factor 2
- Fibroblasts
- Genetic Vectors
- Glycoside Hydrolases
- Glycosylation
- Heparin