Article
Genetic polymorphisms of human ABC transporter ABCG2: development of the standard method for functional validation of SNPs by using the Flp recombinase system.
Journal of experimental therapeutics & oncology - 1 Jan 2006
Tamura Ai, Wakabayashi Kanako, Onishi Yuko, Nakagawa Hiroshi, Tsuji Masahisa, Matsuda Yoichi, Ishikawa Toshihisa
Abstract excerpt
The vector-mediated introduction of cDNA into mammalian cells by calcium phosphate co-precipitation or permeation with lipofectamine is widely used for the integration of cDNA into genomic DNA. However, integration of cDNA into the host's chromosomal DNA occurs randomly at unpredictable sites, and the number of integrated recombinant DNAs is not controllable. To investigate the effect of genetic polymorphisms of...
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