Article
Variation between observed and true Terminal Restriction Fragment length is dependent on true TRF length and purine content.
Journal of microbiological methods - 1 Jul 2003
Kaplan Christopher W, Kitts Christopher L
Abstract excerpt
Terminal Restriction Fragment (TRF) pattern analysis has become a widely used and informative tool for studying microbial communities. Variation between sequence-determined or true TRF length and observed TRF length (TRF drift) has been previously reported and can significantly affect identification of bacterial species using TRF lengths predicted from sequence databases. In this study TRF drift was determined...
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