Article
Evaluating the assignment of alkB terminal restriction fragments and sequence types to distinct bacterial taxa.
Applied and environmental microbiology - 1 May 2013
Giebler Julia, Wick Lukas Y, Schloter Michael, Harms Hauke, Chatzinotas Antonis
Abstract excerpt
Sequence and terminal restriction fragment length polymorphism (T-RFLP) analyses revealed multiple alkB gene copies/cell in soil bacterial isolates and an apparently high genetic mobility among various phylogenetic groups. Identifying alkane degraders by alkB terminal restriction fragments (T-RFs) and sequences is strongly biased, as the phylogenetic trees based on 16S rRNA and alkB gene sequences were highly...
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