Article
Real-time TaqMan PCR for rapid detection and typing of genes encoding CTX-M extended-spectrum beta-lactamases.
Journal of medical microbiology - 1 Jan 2007
Birkett Christopher I, Ludlam Hugo A, Woodford Neil, Brown Derek F J, Brown Nicholas M, Roberts Mark T M, Milner Nicola, Curran Martin D
Abstract excerpt
The prevalence of CTX-M-producing members of the Enterobacteriaceae is increasing worldwide. A novel, multiplex, real-time TaqMan PCR assay to detect and type bla(CTX-M) genes is described which is an improvement on previously described techniques with respect to reduced assay time, elimination of the need for protracted post-PCR processing and the convenience of a single reaction vessel. Based on beta-lactam...
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