Article
Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica - 1 Dec 2007
Monstein H-J, Ostholm-Balkhed A, Nilsson M V, Nilsson M, Dornbusch K, Nilsson L E
Abstract excerpt
Extended-spectrum beta-lactamases (ESBLs) are often mediated by (bla-)SHV, (bla)TEM and (bla)CTX-M genes in Enterobacteriaceae and other Gram-negative bacteria. Numerous molecular typing methods, including PCR-based assays, have been developed for their identification. To reduce the number of PCR amplifications needed we have developed a multiplex PCR assay which detects and discriminates between (bla-)SHV,...
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