Article
Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases.
International journal of antimicrobial agents - 1 Oct 2010
Dhanji Hiran, Doumith Michel, Clermont Olivier, Denamur Erick, Hope Russell, Livermore David M, Woodford Neil
Abstract excerpt
CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.
Topics
- Bacterial Typing Techniques
- DNA, Bacterial
- Epidemics
- Escherichia coli
- Escherichia coli Infections
- Escherichia coli Proteins
- Humans
- Molecular Epidemiology
- Polymerase Chain Reaction
- Polymorphism, Single Nucleotide