Article
Chemistry-based RNA technologies: demonstration of usefulness of libraries of ribozymes and short hairpin RNAs (shRNAs).
Nucleic acids symposium series (2004) - 1 Jan 2005
Miyagishi Makoto, Matsumoto Sahohime, Akashi Hideo, Kawasaki Hiroaki, Fukao Taro, Fukuda Yoko, Sano Masayuki, Kato Yoshio, Takagi Yasuomi, Tanaka Yoshiyuki, Warashina Masaki, Kuwabara Tomoko, Sawata Shinya Y, Ikeda Yutaka, Kawahara Shun-ichi, Sunil Kaul C, Wadhwa Renu, Taira Kazunari
Abstract excerpt
Mechanism of action of hammerhead ribozymes has been investigated and their intracellular activities have been improved. Based on the improved ribozymes and more recently discovered natural RNAi, we have created libraries of both ribozymes and short hairpin RNAs (shRNAs). The introduction of a library of active ribozymes or shRNAs into cells, and the subsequent screening for phenotypic changes, allows the rapid...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.