Article
Quantitative PCR approach to SNP detection and linkage mapping in Caenorhabditis elegans.
BioTechniques - 1 Nov 2006
Shelton Christopher A
Abstract excerpt
I report a method for single nucleotide polymorphism (SNP) detection and linkage mapping in Caenorhabditis elegans using automated oligonucleotide design and fluorescence-based quantitative PCR detection. Nine hundred twenty-three oligonucleotide pairs were designed to produce small products of <150 bp for efficient amplification in a PCR, with one oligonucleotide of each pair overlapping a SNP site at the...
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