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Article

A versatile esterase from Bacillus subtilis: cloning, expression, characterization, and its application in biocatalysis.

Biotechnology journal - 1 Feb 2007

Schmidt Marlen, Henke Erik, Heinze Birgit, Kourist Robert, Hidalgo Aurelio, Bornscheuer Uwe T

Abstract excerpt

An esterase from Bacillus subtilis DSM402 (BS2) was cloned and functionally expressed in E. coli. The enzyme is active up to 50 degrees C, and the V(max) (1449 mM/min) and K(M) values (119 mM) were determined using p-nitrophenyl acetate as substrate. BS2 belongs to the few hydrolases that can act on tertiary alcohols and was therefore used to resolve racemic acetates of selected tertiary alcohols, but also to...

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Identifiers

PMID: 17136743
DOI: 10.1002/biot.200600174

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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