Article
Gene cloning and characterization of a novel thermophilic esterase from Fervidobacterium nodosum Rt17-B1.
Acta biochimica et biophysica Sinica - 1 Apr 2010
Yu Shanshan, Zheng Baisong, Zhao Xinyu, Feng Yan
Abstract excerpt
A bioinformatic screening of the genome of the thermophilic bacterium Fervidobacterium nodosum Rt17-B1 for esterhydrolyzing enzymes revealed a putative bacterial esterase (FNE) encoded by Fond_1301 with typical GDSL family motifs. To confirm its putative esterase function, the FNE gene was cloned, functionally expressed in Escherichia coli, and purified to homogeneity. Recombinant FNE exhibited the highest...
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