Article
In vitro proliferation and cloning of CD3- CD16+ cells from human thymocyte precursors.
The Journal of experimental medicine - 1 Jul 1991
Mingari M C, Poggi A, Biassoni R, Bellomo R, Ciccone E, Pella N, Morelli L, Verdiani S, Moretta A, Moretta L
Abstract excerpt
Purified CD3-4- thymocytes were obtained by depletion of CD3+ and CD4+ cells from fresh thymocyte suspensions. 5-15% of these cells were found to express CD16 antigen, while other natural killer (NK) cell markers were virtually absent. Double fluorescence analysis revealed that 20-40% of thymic C...
Topics
- Antigens, CD
- Antigens, Differentiation
- Antigens, Differentiation, T-Lymphocyte
- Blotting, Northern
- CD3 Complex
- Cell Line
- Cells, Cultured
- Clone Cells
- Cytotoxicity, Immunologic
- Flow Cytometry
- Humans
- Lymphocyte Activation
- Lymphocyte Depletion
- Phenotype
- RNA