Article
In vitro expansion of CD3/TCR- human thymocyte populations that selectively lack CD3 delta gene expression: a phenotypic and functional analysis.
The Journal of experimental medicine - 1 Nov 1990
Poggi A, Biassoni R, Pella N, Paolieri F, Bellomo R, Bertolini A, Moretta L, Mingari M C
Abstract excerpt
Highly purified CD1-3-4-8- human thymocytes were obtained by panning techniques combined with cell depletion with antibody-coated magnetic beads. Most of these cells expressed cytoplasmic CD3 antigen, as assessed by mAbs known to react with the CD3 epsilon chain. After culture with low doses of P...
Topics
- Antigens, CD
- Antigens, Differentiation, T-Lymphocyte
- Base Sequence
- CD3 Complex
- Cell Separation
- Cells, Cultured
- DNA
- Flow Cytometry
- Gene Expression
- Gene Rearrangement, T-Lymphocyte
- Humans
- Lymphokines
- Molecular Sequence Data
- Phenotype