Article
Defining synphenotype groups in Xenopus tropicalis by use of antisense morpholino oligonucleotides.
PLoS genetics - 17 Nov 2006
Rana Amer Ahmed, Collart Clara, Gilchrist Michael J, Smith J C
Abstract excerpt
To identify novel genes involved in early development, and as proof-of-principle of a large-scale reverse genetics approach in a vertebrate embryo, we have carried out an antisense morpholino oligonucleotide (MO) screen in Xenopus tropicalis, in the course of which we have targeted 202 genes expressed during gastrula stages. MOs were designed to complement sequence between -80 and +25 bases of the initiating AUG...
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