Article
Analyses of the interaction between the origin binding domain from simian virus 40 T antigen and single-stranded DNA provide insights into DNA unwinding and initiation of DNA replication.
Journal of virology - 1 Dec 2006
Reese Danielle K, Meinke Gretchen, Kumar Anuradha, Moine Stephanie, Chen Kathleen, Sudmeier James L, Bachovchin William, Bohm Andrew, Bullock Peter A
Abstract excerpt
DNA helicases are essential for DNA metabolism; however, at the molecular level little is known about how they assemble or function. Therefore, as a model for a eukaryotic helicase, we are analyzing T antigen (T-ag) the helicase encoded by simian virus 40. In this study, nuclear magnetic resonance (NMR) methods were used to investigate the transit of single-stranded DNA (ssDNA) through the T-ag origin-binding...
Topics
- Amino Acid Sequence
- Antigens, Viral, Tumor
- DNA Helicases
- DNA Replication
- DNA, Single-Stranded
- DNA, Viral
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Protein Structure, Tertiary
- Replication Origin