Article
Bleomycin-induced DNA lesions at mutational hot spots: implications for the mechanism of double-strand cleavage.
Proceedings of the National Academy of Sciences of the United States of America - 1 Nov 1990
Steighner R J, Povirk L F
Abstract excerpt
Using various end-labeled, defined-sequence DNA substrates, we examined bleomycin-induced damage at several G.C base pairs which correspond to mutational hot spots. The most frequent lesions detected were single-strand breaks and single apurinic/apyrimidinic (AP) sites at the C residue, suggesting that this was the primary site of damage. Strand breaks and AP sites also occurred, but less frequently, at a...
Topics
- Base Composition
- Base Sequence
- Bleomycin
- DNA
- DNA Damage
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Plasmids
- Restriction Mapping