Article
Rye (Secale cereale L.).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Altpeter Fredy
Abstract excerpt
Rye (Secale cereale L.) is one of the most recalcitrant plant species for tissue culture and genetic transformation. Embryogenic rye callus loses its ability to regenerate plants quickly in response to high density of Agrobacterium and other stressors. The cocultivation of Agrobacterium and rye immature embryos in liquid medium facilitated washing of the cultures to avoid Agrobacterium overgrowth and allowed a...
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