Article
Rapid and reproducible Agrobacterium-mediated transformation of sorghum.
Plant cell reports - 1 Aug 2006
Howe Arlene, Sato Shirley, Dweikat Ismail, Fromm Mike, Clemente Tom
Abstract excerpt
A rapid and reproducible Agrobacterium-mediated transformation protocol for sorghum has been developed. The protocol uses the nptII selectable marker gene with either of the aminoglycosides geneticin or paromomycin. A screen of various A. tumefaciens strains revealed that a novel C58 nopaline chromosomal background carrying the chrysanthopine disarmed Ti plasmid pTiKPSF(2), designated NTL(4)/Chry5, was most...
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