Article

Agrobacterium tumefaciens-mediated genetic transformation of rye (Secale cereale L.)

2003-04-01

Abstract excerpt

A system for the genetic transformation of rye by co-cultivation with Agrobacterium tumefaciens is described. A total of 45 independent transgenic plants were regenerated with a transformation efficiency of 1 to 4% of the inoculated explants. The co-cultivation of Agrobacterium-strain AGL(0), harboring plasmid pJFnptII and rye immature embryos in liquid medium allowed a high throughput and facilitated washing of t...

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Literature Corpus work: cf37fea6-c092-5508-a3c7-6ede17e223a0
DOI: 10.1023/a:1022876318276

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