Article
Wheat (Triticum aestivum L.).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Wan Yuechun, Layton Jeanne
Abstract excerpt
This chapter describes a procedure for Agrobacterium-mediated wheat transformation. Freshly isolated immature embryos, precultured immature embryos, or embryogenic calli are inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the beta-glucur...
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