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Article

Using single loxP sites to enhance homologous recombination: ts mutants in Sec1 of Dictyostelium discoideum.

PloS one - 8 Aug 2007

Bretscher Mark S, Clotworthy Margaret

Abstract excerpt

BACKGROUND: Dictyostelium discoideum amoebae are haploid and, as they share many features with animal cells, should be an ideal creature for studying basic processes such as cell locomotion. Isolation of mutants in this amoeba has largely been limited to non-essential genes: nsfA-the gene for NEM-sensitive factor-remains the only essential gene for which conditional (ts) mutants exist. These ts mutants were...

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PMID: 17684569
DOI: 10.1371/journal.pone.0000724
PMCID: PMC1933600

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