Article
Reactivity of monoclonal antibody E5 with endotoxin. I. Binding to lipid A and rough lipopolysaccharides.
Circulatory shock - 1 Sept 1992
Wood D M, Parent J B, Gazzano-Santoro H, Lim E, Pruyne P T, Watkins J M, Spoor E S, Reardan D T, Trown P W, Conlon P J
Abstract excerpt
The murine IgM monoclonal antibody (mAb) E5 was produced by a hybridoma derived from spleen cells of a mouse immunized with the J5 rough mutant of Escherichia coli O111:B4. In a multicenter randomized placebo-controlled clinical trial, E5 has been shown to reduce significantly the mortality and morbidity of patients with Gram-negative sepsis. The characteristics of E5 binding to endotoxin were studied in vitro....
Topics
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Antigens, Bacterial
- Binding Sites, Antibody
- Binding, Competitive
- Endotoxins
- Enzyme-Linked Immunosorbent Assay
- Escherichia coli
- Hybridomas