Article
Use of colloidal silica (Sepracell-MN) for enrichment of dendritic cells from human peripheral blood: comparison with other methods.
Journal of leukocyte biology - 1 Jul 1990
Chehimi J, Starr S E, Kawashima H, Hassan N F, Douglas S D, Bandyopadhyay S
Abstract excerpt
Three methods are described for enrichment of dendritic cells from human peripheral blood. In method 1, mononuclear cells were incubated in plastic tissue culture flasks for two h. Nonadherent cells were removed. Adherent cells were washed to remove floating cells and incubated for 14 h at 37 degrees C in 5% CO2. Carbonyl iron was added, and the flasks were incubated for another 2 h. Nonadherent cells were...
Topics
- Antibodies
- Antigens, Differentiation
- Antigens, Differentiation, Myelomonocytic
- B-Lymphocytes
- Blood Cells
- CD5 Antigens
- Cell Adhesion
- Cells, Cultured
- Centrifugation
- Colloids
- Complement System Proteins