Article
Base sequence specificity of a monoclonal antibody binding to (6-4)photoproducts.
Mutation research - 1 May 1990
Matsunaga T, Mori T, Nikaido O
Abstract excerpt
The DNA base sequence specificity of the 64M-1 monoclonal antibody, which recognizes ultraviolet (UV)-induced (6-4)photoproducts, was characterized. The 64M-1 antibody strongly bound to UV-poly(dU) as well as to UV-poly(dT), and weakly to UV-poly(dC), UV-poly(me5dC) and UV-poly(rU). A competitive inhibition assay using UV-oligo(dT)8, UV-oligo(dTdC)4, UV-oligo(dC)8, UV-PvuI linker (GCGATCGC) and UV-PvuII linker...
Topics
- Animals
- Antibodies, Monoclonal
- DNA
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- Mice
- Mice, Inbred BALB C
- Mutation
- Oligonucleotides
- Polynucleotides
- Pyrimidine Dimers
- Sensitivity and Specificity