Analytical- and preparative-scale separation of molecular variants of alpha-fetoprotein by anion-exchange chromatography on Monobead resins.

doi:10.1016/s0378-4347(00)83379-4

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Article

Analytical- and preparative-scale separation of molecular variants of alpha-fetoprotein by anion-exchange chromatography on Monobead resins.

Journal of chromatography - 26 Jan 1990

Van Oers N S, Boismenu R, Cohen B L, Murgita R A

Abstract excerpt

A rapid and reliable purification procedure is described that is useful for both analytical detection and quantitative recovery of milligram amounts of individual molecular variants of mouse alpha-fetoprotein (AFP). The appropriate separation conditions were developed with an analytical-size Mono...

Topics

Animals
Chromatography, Ion Exchange
Genetic Variation
Immunoblotting
Isoelectric Focusing
Mice
Resins, Plant
alpha-Fetoproteins

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