Ion-exchange high-performance liquid chromatographic separation of protein variants and isoforms on MCI GEL ProtEx stationary phases.

Abstract excerpt

Protein variants and isoforms were successfully separated on MCI GEL ProtEx ion-exchange HPLC columns. There was no irreversible adsorption of proteins, and sample proteins were quantitatively recovered. Species variants of cytochrome c (bovine, horse and rabbit) were completely separated on a sulfopropyl (ProtEx-SP) stationary phase in a gradient system. Diethylaminoethyl (ProtEx-DEAE) phase was determined to be...

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