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Article

19F NMR studies of the native and denatured states of green fluorescent protein.

Journal of the American Chemical Society - 23 Aug 2006

Khan Farid, Kuprov Ilya, Craggs Timothy D, Hore P J, Jackson Sophie E

Abstract excerpt

Biosynthetic preparation and (19)F NMR experiments on uniformly 3-fluorotyrosine-labeled green fluorescent protein (GFP) are described. The (19)F NMR signals of all 10 fluorotyrosines are resolved in the protein spectrum with signals spread over 10 ppm. Each tyrosine in GFP was mutated in turn to phenylalanine. The spectra of the Tyr --> Phe mutants, in conjunction with relaxation data and results from (19)F...

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PMID: 16910667
DOI: 10.1021/ja060618u

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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