Article
Multitypic hepatitis C virus infection identified by real-time nucleotide sequencing of minority genotypes.
Journal of clinical microbiology - 1 Aug 2006
Buckton Andrew J, Ngui Siew-Lin, Arnold Catherine, Boast Katie, Kovacs Joanne, Klapper Paul E, Patel Bharat, Ibrahim Imad, Rangarajan Savita, Ramsay Mary E, Teo Chong-Gee
Abstract excerpt
The prevalence of concurrent multitypic hepatitis C virus (HCV) infection is uncertain. A sensitive and specific approach to identifying minority HCV genotypes in blood is presented. Following serum extraction and reverse transcription PCR to amplify cDNA originating from the viral 5' noncoding region, the amplified product mixture was treated with genotype-specific restriction endonuclease to digest the dominant...
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