Article
Random insertion of a TetR-inducing peptide tag into Escherichia coli proteins allows analysis of protein levels by induction of reporter gene expression.
Applied and environmental microbiology - 1 Aug 2006
Schlicht Maximilian, Berens Christian, Daam Janko, Hillen Wolfgang
Abstract excerpt
The insertion element InsTipalpha was constructed to generate protein expression data. It randomly fuses the TetR-inducing peptide Tip to the affected reading frame. Fusion protein expression is quantified by Tet-regulated reporter gene expression. The expression patterns of tagged Escherichia coli genes fully agree with published data from transcriptional fusions or microarrays, validating the Tip tag approach.
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